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. 2017 Jun 30;7(2):299–312. doi: 10.15171/apb.2017.036

Figure 3.

Figure 3

Engineering and verification of pCMX2.5-scFv 183-H12-5C-hIgG1-Fc vector. Schematic representation of expression vector pCMX2.5-scFv 183-H12-5C-hIgG-Fc (A). scFv 183-H12-5C gene was restricted from pHEN2-scFv 183-H12-5C used NcoI/NotI enzymes, gel purified and cloned at NcoI/NotI restriction sites in restricted pCMX2.5-hIgG1-Fc vector (B). Colony PCR used CMV-Seq-F/scFv 183-H12-5C NotI-R primers; Presence of ~860 bp band in most of the clones suggests the presence of scFv 183-H12-5C in a pCMX2.5-scFv 183-H12-5C-hIgG1-Fc plasmid vector (C). Restriction analysis of pCMX2.5-scFv 183-H12-5C-hIgG1-Fc with NcoI/NotI (5.4 kb vector backbone + 855 bp insert) (D).