Skip to main content
. 2017 Jun 30;7(2):299–312. doi: 10.15171/apb.2017.036

Figure 7.

Figure 7

Kinetic analysis of HIV-1 infectivity on Jurkat T cell-scFv 183-H12- 5C infected with HIV-1 NL4-3 virus. To express intracellularly, pCDNA3.3-scFv 183-H12-5C plasmid vector was electroporated into Jurkat T and expressed constitutively under CMV promoter. Transiently expressing scFv 183-H12-5C-6His Jurkat T cells were infected with HIV-1 (NL4.3) at MOI 0.1 and culture supernatants were collected every 24 h and used in MAGI infectivity assay (A). Percentage of HIV-1 inhibition in Jurkat T cell transiently expressing scFv 183-H12-5C (B). The results are representative of three independent experiments each done in duplicate.