Table 1. Oligonucleotides used for engineering of pcDNA3.3-scFv 183-H12-5C-6His. Restriction enzyme (italic) and its recognition site underline.

Set	Primer name	Primer sequence (5'⟶3')
For engineering of pcDNA3.3-scFv 183-H12-5C-6His
1	pcDNA3.3-NotI6H-F	AACGGGCGGCCGCACATCATCATCACCATCACTGAAGGGTTCGATCCCTACCGG
	pcDNA3.3-SfiI-R	CCATGGCCGGCTGGGCCGCAGGGTTCGATCCTCTAGAGTCCGG
2	scFv 183-H12-5C-SfiI-F	CCTGCGGCCCAGCCGGCCATGGCCCAGGTCCAACTGC
	scFv 183-H12-5C-NotI-R	GATGTGCGGCCGCCCGTTTCAGTTCCAGTTTGGTC
For colony PCR of scFv 183-H12-5C in pCDNA3.3 plasmid backbone
1	CMV-F	CGCAAATGGGCGGTAGGCGTG
	scFv 183-H12-5C-NotI-R	GATGTGCGGCCGCCCGTTTCAGTTCCAGTTTGGTC