Table 2. Oligonucleotides used for engineering of pcDNA3.3-mu-IgGk-scFv 183-H12-5C-6His. Restriction enzyme (italic) and its recognition site underline.

Set	Primer name	Primer sequence (5'⟶3')
For engineering of pcDNA3.3-mu-IgGkappa-scFv 183-H12-5C-6His
1	scFv 183-H12-5C-SfiI-F1	CCTGCGGCCCAGCCGGCCATGGCCCAGGTCCAACTGC
	CMV-BsiWI-R1	CCATCGTACGTCGATCCTCTAGAGTCCGGAG
2	Mu IgG k-F	GTACGATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCGGCCCAGC
	Mu IgG k-R	GGGCCGCGTCACCAGTGGAACCTGGAACCCAGAGCAGCAGTACCCATAGCAGGAGTGTGTCTGTCTCCATC
For colony PCR of murine-IgG kappa-scFv 183-H12-5C in pCDNA3.3 plasmid backbone
1	BsiWI-MuIgGk-F	CGTACGATGGAGACAGACACACTCCTG
	6His-A-Seq-R	CCTTCAGTGATGGTGATGATGATGTGC