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. 2017 Jun 30;7(2):299–312. doi: 10.15171/apb.2017.036

Table 3. Oligonucleotides used for verification of pCMX2.5-scFv 183-H12-5C-hIgG1-Fc. Restriction enzyme (italic) and its recognition site underline.

Set Primer name Primer sequence (5'⟶3')
For colony PCR of scFv 183-H12-5C in pCMX2.5 plasmid backbone
1 CMV-F CGCAAATGGGCGGTAGGCGTG
scFv 183-H12-5C-NotI-R GATGTGCGGCCGCCCGTTTCAGTTCCAGTTTGGTC