Table 3. Oligonucleotides used for verification of pCMX2.5-scFv 183-H12-5C-hIgG1-Fc. Restriction enzyme (italic) and its recognition site underline.
Set | Primer name | Primer sequence (5'⟶3') |
For colony PCR of scFv 183-H12-5C in pCMX2.5 plasmid backbone | ||
1 | CMV-F | CGCAAATGGGCGGTAGGCGTG |
scFv 183-H12-5C-NotI-R | GATGTGCGGCCGCCCGTTTCAGTTCCAGTTTGGTC |