Figure 4.

Neurotransmitter and the receptor regulated following spastic paralysis. (A-C) ELISA was performed to determine the expression of Glycine (A), GABA (B) and Glutamic (C) in the ventral horn of spinal cord area of control group, and 10 min, 3 h, 6 h, 24 h and 72 h post reperfusion group. (D-F) The expression of Gly α1, GABBR2 and NR1 in the ventral horn of spinal cord area was also determined by Western blotting. GAPDH was used as a loading control. The relative expression was analyzed. (n=3, *P<0.05, **P<0.01, compared with control group).