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. 2017 Jul 15;9(7):3452–3461.

Figure 4.

Figure 4

AQP4 knockdown prevented the protective effect of miR-130b. A: Determination of AQP4 siRNA efficiency by Western blotting; β-actin was used as the internal control, and the average band intensity is represented as a histogram. B: LDH release in OGD-treated primary astrocytes transfected with AQP4 siRNA and miR-130b mimic, AQP4 siRNA and miR-130b inhibitor or AQP4 siRNA alone. ***P < 0.001 vs. NC group. NC: negative control. C: LDH release from OGD-treated primary astrocytes transfected with AQP4 siRNA and miR-130b mimic, AQP4 siRNA and miR-130b inhibitor or AQP4 siRNA alone by ELISA. D: Cell apoptosis in OGD-treated primary astrocytes transfected with AQP4 siRNA and miR-130b mimic, AQP4 siRNA and miR-130b inhibitor or AQP4 siRNA alone. Apoptosis was determined by flow cytometry with Annexin V-FITC/PI kit. Cells in the B2 and B4 quadrants represented the apoptotic cells.