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Phenotypic characterization of MSCs isolated from MDS patients and healthy donors. Total nucleated cells of bone marrows were obtained using Ficoll-Hypaque separation and plated into 25-cm2 culture flasks inHuman Mesenchymal Stem Cell Growth Medium as described in Materials and Methods. Cells from the third passage were used for flow cytometry analysis. Single live cells were gated. Representative plots from one MDS sample and one control sample were shown.
