Figure 1.

LncRNA H19 was overexpressed in TSCC tissues and significantly associated with poor prognosis. A. Insitu hybridization was performed to detect H19 expression in TSCC tissues with and without metastasis and in adjacent tongue tissues. The carcinoma with metastasis had a higher expression of H19 than that in the carcinoma without metastasis. B. The relative expression of H19 was examined by qRT-PCR in 50 TSCC tissues and matched adjacent non-cancerous tissues. The relative gene expression was normalized to that of GAPDH. C. The relative expression of H19 was much higher at advanced pathological stages in TSCC patients. D. The relative expression of H19 was analyzed in tissues from TSCC patients with or without lymph node metastasis. E. Kaplan-Meier overall survival curves were generated for patients with different H19 expression levels. The higher expression of H19 had a much shorter lifespan. Data represent the mean ± SD. #P<0.05, ##P<0.01, ###P<0.001.