Figure 4.

EZH2 was upregulated in TSCC tissues and closely associated with poor prognosis. A. Immunohistochemistry was performed to detect the expression of EZH2 in TSCC tissues with and without metastasis, and in adjacent normal tongue tissues. The carcinoma with metastasis had a higher expression of EZH2 than that in the carcinoma without metastasis. B. The relative expression of EZH2 was examined by qRT-PCR in 50 TSCC tissues and matched adjacent non-cancerous tissues. The relative gene expression was normalized to GAPDH. C. The relative expression of EZH2 was much higher at advanced pathological stages in TSCC patients. D. The relative expression of EZH2 was analyzed between TSCC patients with or without lymph node metastasis. E. Kaplan-Meier overall survival curves were generated for patients with different EZH2 expression levels. The higher expression of EZH2 had a much shorter lifespan. Data represent the mean ± SD. #P<0.05, ##P<0.01, ###P<0.001.