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High-performance liquid chromatography (HPLC) analysis of puerarin in kudzu extract. A: The HPLC graph of standard puerarin; B: Calibration curve of standard puerarin; C: The HPLC graph of puerarin in kudzu extract. HPLC parameters: C18 shim-pack VP-ODS (150 L × 4.6 8,052,494); mobile phase: methanol-water (25:75); column temperature: 40°C; flow velocity: 0.8 ml/min; detection wavelength: 250 nm.
