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. Author manuscript; available in PMC: 2017 Jul 26.
Published in final edited form as: Cell Rep. 2017 Feb 28;18(9):2077–2087. doi: 10.1016/j.celrep.2017.02.004

Figure 4. BHB inhibits inflammasome priming and assembly.

Figure 4

(A) Western blot of culture supernatants from LPS-primed WT and Caspase 1/11−/− neutrophils after ATP, Silica, or ceramide stimulation as indicated. (B, C) Elastase and Cathepsin G activity; n=4. (D–F) Culture supernatants were analyzed for neutrophil IL-1β secretion by Western blot after indicated treatments. (G) Total and acetylated H3 expression in cell lysates. (H) NFκB phosphorylation in neutrophil cell lysates. (I) IL-1β secretion from neutrophils derived from mouse model of FCAS bearing activating mutation of Nlrp3. For all blots, each sample is pooled from at least n=4 mice per experiment. Each blot is representative of at least two independent experiments. See also Figure S4.