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. 2017 Jul 25;8(4):e00715-17. doi: 10.1128/mBio.00715-17

FIG 8 .

FIG 8 

Effects of carbon and nitrogen sources and oxygen concentrations on the expression of the reb operon under free-living conditions. Mutant reb-lacZ and reb-lacZ ΔpraR strains were grown in medium containing 50 mM pyruvate, citrate, 2-oxoglutarate (2OG), succinate, fumarate, or malate as carbon sources and 10 mM NH4+ or NO3 as nitrogen sources or without a nitrogen source (−N) under aerobic (21% O2) or microaerobic (3% O2) conditions. Initial OD600 values of cultures were adjusted to 0.02 for pyruvate, succinate, fumarate, and malate media with NH4+ or NO3 supplementation, 0.1 for supplementation with 2OG and NH4+ or NO3, and 0.2 for supplementation with citrate medium and NH4+ or NO3 or nitrogen-deficient media. After incubation at 26°C for 24 h, β-galactosidase activities were measured. Data are presented as means ± standard deviations from three replicate cultures. Different letters indicate significant differences (P < 0.05, Tukey-Kramer).