TABLE 2 .
Plasmids and yeast strains used in this study
| Plasmid or strain | Description or relevant genotype | Reference |
|---|---|---|
| Plasmids | ||
| pCM252 | TRP1 VP16::TetR' TetO7pr | 25 |
| pAH12 | pCM252 TetO7pr::relA | This work |
| pAH36 | pCM252 TetO7pr::ybbP | This work |
| pAH71 | pCM252 TetO7pr::dgcA | This work |
| pAH112 | pRS-HIS3-MET15 TetR::SSN6 | This work |
| pAH134 | pRS-LEU2-URA3 VP16::TetR' TetO7pr | This work |
| pAH135 | pRS-LEU2-URA3 VP16::TetR' TetO7pr::relA | This work |
| pAH136 | pRS-LEU2-URA3 VP16::TetR' TetO7pr::ybbP | This work |
| pAH137 | pRS-LEU2-URA3 VP16::TetR' TetO7pr::dgcA | This work |
| pAH139 | pRS-LEU2-URA3 VP16::TetR' TetO7pr::TDH3pr::MESH1 | This work |
| pAH142 | pRS-LEU2-URA3 VP16::TetR' TetO7pr::relA TDH3pr::MESH1 | This work |
| Strains | ||
| CML282 | BMA64-1A MATa leu2-3 ade2-1 trp1-1 ura3-1 can1-100 his3-11,15 LEU2::(TetR::SSN6) | 25 |
| CML282-252 | CML282 with plasmid pCM252 | |
| CML282-12 | CML282 with plasmid pAH12 | This work |
| CML282-36 | CML282 with plasmid pAH36 | This work |
| CML282-71 | CML282 with plasmid pAH71 | This work |
| BY4741 | MATa ura3Δ0 leu2Δ0 met15Δ0 his3Δ1 | |
| BY4741-112 | BY4741 with integrated plasmid pAH112 (his3Δ1::pAH112 ura3Δ0 leu2Δ0) | |
| BY4741-112-134 | BY4741-112 with centromeric plasmid pAH134 (prototroph) | This work |
| BY4741-112-135 | BY4741-112 with centromeric plasmid pAH135 (prototroph) | This work |
| BY4741-112-136 | BY4741-112 with centromeric plasmid pAH136 (prototroph) | This work |
| BY4741-112-137 | BY4741-112 with centromeric plasmid pAH137 (prototroph) | This work |
| BY4741-112-139 | BY4741-112 with centromeric plasmid pAH139 (prototroph) | This work |
| BY4741-112-142 | BY4741-112 with centromeric plasmid pAH142 (prototroph) | This work |
| Y7092 | MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 can1Δ::STE2pr-Sp_his5 lyp1Δ | 24 |
| Y7092-112 | Y7092 with integrated plasmid pAH112 [his3Δ1::pAH112(his3Δ) ura3Δ0 leu2Δ0] | This work |
| Y7092-112-134 | Y7092-112 with centromeric plasmid pAH134 [his3Δ1::pAH112(his3Δ)] | This work |
| Y7092-112-135 | Y7092-112 with centromeric plasmid pAH135 [his3Δ1::pAH112(his3Δ)] | This work |
| Y7092-112-136 | Y7092-112 with centromeric plasmid pAH136 [his3Δ1::pAH112(his3Δ)] | This work |
| Y7092-112-137 | Y7092-112 with centromeric plasmid pAH137 [his3Δ1::pAH112(his3Δ)] | This work |
| BY4741 mutant collection | MATa ura3Δ0 leu2Δ0 met15Δ0 his3Δ1::KANMX4 | 69 |