FIG 1.

Recombinant BosR contains a structural Zn site. Removal of Zn from recombinant BosR requires EDTA chelation under a denaturing condition. The Mn, Fe, Cu, and Zn levels in BosR were determined by ICP-SFMS and are shown as number of atoms per BosR dimer. BosR was analyzed as isolated (untreated) or after being mock treated or treated with 10 mM EDTA, either alone or in combination with urea (2 M) or SDS (0.1%). Data represent mean (±standard error of the mean [SEM]) of three readings of each sample. Values of treated samples were compared with those of the untreated sample using a two-way ANOVA, followed by Bonferroni posttest. Only P values of statistical significance are indicated: *, P < 0.05; ***, P < 0.001.