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. 2017 Jul 25;199(16):e00276-17. doi: 10.1128/JB.00276-17

FIG 3.

FIG 3

Cu(II) and Zn(II) inhibit BosR DNA-binding activity. The effect of Mn(II) (A), Fe(II) (B), Cu(II) (C), or Zn(II) (D) on BosR binding to a fluorescently labeled DNA probe was determined by measuring changes in fluorescence anisotropy (ΔFA). See Materials and Methods for details. The curves were fitted for one-site, specific binding with Hill slope using GraphPad Prism 6. R2 was ≥0.99 for all curves.