TABLE 1.
S. marcescens strains and plasmids
| Name | Relevant genotype | Description | Reference or source |
|---|---|---|---|
| S. marcescens | |||
| UMH9 | Wild type | Bloodstream infection isolate | 15 |
| phlAB | ΔphlAB::nptII | 1,519-bp internal deletion of phlAB locus, insertion of 1,496-bp nptII-containing fragment from pKD4 | This study |
| cysE | cysE::Tn | Insertion at position 138 of 822-bp ORF | This study |
| cyaA | cyaA::Tn | Insertion at position 218 of 2,553-bp ORF | This study |
| cyaA | cyaA::Tn | Insertion at position 104 of 2,553-bp ORF | This study |
| crp | crp::Tn | Insertion at position 123 of 633-bp ORF | This study |
| fliP | fliP::Tn | Insertion at position 471 of 762-bp ORF | This study |
| fliJ | fliJ::Tn | Insertion at position 164 of 447-bp ORF | This study |
| Plasmids | |||
| pKD4 | Source of kanamycin resistance gene | 40 | |
| pSIM19 | Recombineering plasmid | 41 | |
| pBBR1MCS-5 | Genetic complementation plasmid, gentamicin resistant | 42 | |
| pBB1 | phlAB+ | phlAB locus with native promoter sequence ligated to pBBR1MCS-5 | This study |
| pBB2 | cysE+ | cysE ORF with predicted promoter sequence ligated to pBBR1MCS-5 | This study |