TABLE 2.
Analytical performance of the PathoNostics AsperGenius species assay for testing of A. fumigatus genomic DNA extracted from plasma samples by use of a bioMérieux EasyMag instrument
| DNA template vol (μl) | Fungal load (genomes/0.5-ml sample) | PathoNostics AsperGenius target |
|||||||
|---|---|---|---|---|---|---|---|---|---|
|
A. fumigatus |
Aspergillus spp. |
A. terreus |
Internal control |
||||||
| No. of positive results/total no. of samples | Mean Cq value (SD) | No. of positive results/total no. of samples | Mean Cq value (SD) | No. of positive results/total no. of samples | Mean Cq value (SD) | No. of positive results/total no. of samples | Mean Cq value (SD) | ||
| 5 | 10,000 | 1/1 | 24.72 | 1/1 | 23.86 | 1/1 | 41.95 | 1/1 | 29.68 |
| 1,000 | 1/1 | 27.93 | 1/1 | 26.55 | 0/1 | 1/1 | 32.25 | ||
| 500 | 1/1 | 28.85 | 1/1 | 27.23 | 0/1 | 1/1 | 31.67 | ||
| 100 | 1/1 | 31.26 | 1/1 | 29.43 | 0/1 | 1/1 | 32.43 | ||
| 50 | 3/3 | 33.24 (0.98) | 3/3 | 30.75 (0.81) | 1/3 | 39.39 | 3/3 | 33.33 (0.67) | |
| 25 | 3/3 | 34.31 (1.79) | 3/3 | 31.20 (0.61) | 1/3 | 42.10 | 3/3 | 33.57 (0.80) | |
| 10 | 5/5 | 37.5 (1.63) | 5/5 | 32.5 (0.41) | 0/5 | 5/5 | 31.86 (0.61) | ||
| 5 | 5/5 | 38.0 (2.66) | 5/5 | 33.28 (0.73) | 0/5 | 5/5 | 33.27 (0.82) | ||
| 1 | 0/5 | 1/5 | 35.35 | 1/5 | 43.90 | 5/5 | 33.48 (1.00) | ||
| 0 | 0/5 | 0/5 | 0/5 | 5/5 | 31.83 (1.56) | ||||
| 10 | 10 | 1/1 | 35.82 | 1/1 | 34.46 | 0/5 | 1/1 | 33.15 | |
| 5 | 5/5 | 38.65 (1.90) | 5/5 | 35.80 (1.15) | 0/5 | 5/5 | 30.56 (1.00) | ||
| 1 | 3/5 | 40.67 (2.14) | 3/5 | 36.94 (1.45) | 0/5 | 5/5 | 31.59 (1.77) | ||
| 0 | 0/5 | 0/5 | 0/5 | 5/5 | 32.60 (1.25) | ||||