Figure 5.

Dual inhibition of Akt and c‐Met induces apoptosis and autophagy of sorafenib‐resistant HCC cells. Huh7‐SR and HepG2‐SR cells were incubated for 48 h with capmatinib (2 nm), or MK2206 (1 μm), or the combination. (A, B) Cell apoptosis (%) was measured by cytometry. (C) Representative images were taken from cells stained with annexin V/propidium iodide (magnification ×100). (D) Representative images were taken from acridine orange‐stained cells (magnification ×400). (E) The fold change of acridine orange fluorescence intensity (FL3) versus untreated controls was calculated. The FL3 in untreated controls was defined as 1. (F) Lysates of the above Huh7‐SR cells were immunoblotted. ‘#’ (P < 0.05) and ‘##’ (P < 0.001) indicate a significant increase from controls. ‘ϕϕ’ (P < 0.001) vs. capmatinib alone; ‘†’ (P < 0.05) and ‘††’ (P < 0.001) vs. MK2206 alone.