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. 2017 Apr 4;11(5):584–594. doi: 10.1002/1878-0261.12056

Figure 3.

Figure 3

SDS/PAGE, western blot analysis of the anti‐human CD19 immunotoxins. (A) SDS/PAGE analysis (4–12% NuPAGE, Thermo Fisher Scientific); (B) western blot analysis using a mouse anti‐His mAb (clone#: 4A12E4, Thermo Fisher Scientific); (C) western blot analysis using a mouse antidiphtheria toxin mAb (clone# 3B6, Meridian). Lane 1: protein marker; Lanes 2–3: monovalent anti‐human CD19 immunotoxin [DT390‐scFv (FMC63), 70.09 kDa]; Lanes 4–5: bivalent anti‐human CD19 immunotoxin [DT390‐BiscFv (FMC63), 96.95 kDa]; Lanes 6–7: single‐chain foldback diabody anti‐human CD19 immunotoxin (95.69 kDa).