Figure 2.

SK1 expression induces a glycolytic switch in A2780 ovarian cancer cells. A2780 mock and SK1 cells were cultured in growing medium or serum‐starved for 24 h. (A) Box plots: the relative concentration levels of the indicated metabolites in each group were calculated by integrating the signal area in the respective ¹H‐NMR spectra. Changes in metabolite levels caused by SK1 overexpression were statistically significant by paired Wilcoxon test, *P < 0.05. (B) [¹⁴C]‐glucose uptake normalized on protein content. The increase in glucose uptake induced by SK1 expression was statistically significant by Student's t‐test, *P < 0.05. (C) Expression levels of GLUT1 and GLUT3 transporters by WB. Equally loaded protein was checked by expression of β‐isoform of actin. A blot representative of three independent experiments with analogous results is shown. Bar plots represent band intensity of GLUT1 and GLUT3 normalized to β‐actin and reported as mean ± SEM of three independent experiments, ‐fold change over control. The effect of SK1 expression was statistically significant by Student's t‐test, *P < 0.05. (D) MCT1 and MCT4 expression by WB. Equally loaded protein was checked by expression of β‐isoform of actin. A blot representative of three independent experiments with analogous results is shown. Bar plots represent the densitometric analysis of at least three independent experiments. Data are the mean ± SEM and are reported as protein expression normalized to β‐actin, ‐fold change over control (set as 1). The effect of SK1 expression was statistically significant by Student's t‐test, *P < 0.05.