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. 2017 Mar 6;11(4):388–404. doi: 10.1002/1878-0261.12043

Figure 2.

Figure 2

Knockdown of CNOT1 inhibits osteosarcoma cell proliferation in vitro. (A–C) The mRNA and protein expression levels were validated after si‐CNOT1 and si‐NC transfection by qRT‐PCR and western blotting in MNNG/HOS, MG63, and U2OS cells. (D–F) CCK‐8 assay was performed after siRNA transfection. (G–I) Cell cycle assays were performed for CNOT1‐silenced osteosarcoma cells and control cells. (J–L) colony forming assay for CNOT1‐silenced osteosarcoma cells and control cells. Data are representative of the results from three independent experiments. *P < 0.05.