Skip to main content
. 2017 Mar 6;11(4):388–404. doi: 10.1002/1878-0261.12043

Figure 6.

Figure 6

Knockdown of CNOT1 inhibits osteosarcoma cell proliferation through LMNA. (A,C) Representative blots of LMNA after transfection with pLVX‐Pruo‐Flag‐LMNA plasmid plus si‐CNOT1 or si‐NC. β‐Actin was used as an internal control. (B,D) CCK‐8 assay was used to detect tumor cell proliferation after transfection with pLVX‐Pruo‐Flag‐LMNA plasmid plus si‐CNOT1 or si‐NC. (E, F, G, H) colony forming assay was performed after transfection with pLVX‐Pruo‐Flag‐LMNA plasmid plus si‐CNOT1 or si‐NC. (I, J) Representative blots of GLI1, PTCH1, and PTCH2 after transfection with pLVX‐Pruo‐Flag‐LMNA plasmid plus si‐CNOT1 or si‐NC. β‐Actin was used as an internal control.