Figure 5.

Drug‐induced PD‐L1 expression in primary bone marrow stromal cells from lymphoma patients. (A) Morphology of primary bone marrow stromal cells. Fresh bone marrow specimens were processed and stromal cells were cultured as described in Materials and methods. The stromal cells were cultured in fresh medium without or with 0.01 μm ADM for 24 h, and cell morphology was examined using a light microscope (×40). (B) Effect of ADM on the expression of PD‐L1 and ERK, analyzed by western blotting. (C) Effect of ADM (0.01 μm, 12 h) on the expression of PD‐L1, analyzed by immunohistochemistry as described in Materials and methods.