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. 2009 Jul 29;3(5-6):451–463. doi: 10.1016/j.molonc.2009.07.004

Figure 1.

Figure 1

TGIF has a linear effect on HL60 cell proliferation. (A) Lentiviral shRNA‐mediated TGIF knockdown in HL60 cells. Several clones were analyzed and the degree of knockdown was confirmed with both relative real‐time PCR and Western blot analysis (TGIF knockdown Western blot was exposure time was ∼60s while the TGIF over‐expression Western blot time was ∼10s; unequal times were used for better visualization of the respective bands). Data from one such clone is shown. The real‐time analysis was repeated at least 3 times and the results of one representative experiment are shown. Error bars represent SEM. Proliferation inhibition of HL60 cells with TGIF knockdown. (B) TGIF over‐expression (with plasmid p721) resulted in increased proliferation. (C) Proliferation analysis was done in triplicate with manual counting with trypan blue exclusion. Each sample of the triplicate was counted at least 3 times at each time point. X‐axis represents time in hours, Y‐axis shows the number of cells per ml. Several clones were analyzed and compared with HL60 cells and cells transduced with non‐mammalian shRNA. Proliferation analysis was repeated twice and the results of one such analysis is shown. Error bars represent SEM. P values were calculated using students t‐test with the help of Prism 5 statistical suite.