FIG 6.

Reversion of S. aureus hemY::IS256-spc to the wild-type colony phenotype. (A) S. aureus hemY::IS256-spc (black) reverting to the large-colony phenotype (gray) during daily serial passages in TSB medium without antibiotic. (B and C) Same experiment as for panel A performed in TSB containing antibiotic (150 μg/ml of spectinomycin) in the first culture on day 0 only (B) and in TSB containing 150 μg/ml of spectinomycin from day 0 to day 4 (C). Error bars indicate the standard deviations from at least 3 experiments.