TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| Staphylococcus aureus strains | ||
| HG001 | rsbU-repaired laboratory strain NCTC 8325 | 59 |
| SA137/93A | Clinical VISA/MRSA isolate | 18 |
| SA137/93G | Spontaneous mutant of clinical VISA isolate SA137/93A; ΔSCCmec (Mets) | 18 |
| SA1450/94 | MRSA, Northern German epidemic strain; mutS::IS256 | 18 |
| HG001 hemY::IS256-spc | hemY::IS256-spc insertion mutant; DR: TATTAGGAT | This study |
| HG001 G2 | Intergenic IS256-spc insertion mutant (SAOUHSC_0967/SAOUHSC_0968); DR: TTTTTAGC | 7 |
| HG001 G7 | Intergenic IS256-ermB insertion mutant (SACOL0573/gltX) | This study |
| HG001 W5 | rsbU::IS256-spc insertion mutant; DR: TTTAATTA | 7 |
| HG001 guaA::IS256-ermB | guaA::IS256-ermB insertion mutant; DR: CAATTCGG | This study |
| HG001 R1 | Revertant of HG001 hemY::IS256-spc | This study |
| Plasmids | ||
| pBT2 | E. coli-S. aureus shuttle vector with thermosensitive origin of replication for Gram-positive bacteria; cat | 7 |
| pBT2-ermB | pBT2 harboring the erythromycin resistance-mediating cassette ermB as insert | 26 |
| pA3 | pBT2 carrying a recombinant IS256 element containing an erythromycin resistance-mediating cassette | 26 |
| pA6 | pBT2 carrying a recombinant IS256 element containing a spectinomycin resistance-mediating cassette, spc from S. aureus Mu50 (GenBank accession no. BA000017.4) | 7 |
| pMAD | E. coli-S. aureus shuttle vector with a thermosensitive origin of replication for Gram-positive bacteria; ermC bgaB | 60 |
a
VISA, vancomycin-intermediate S. aureus; DR, direct repeat.