TABLE 4.
Oligonucleotide | Sequence (5′–3′)a |
---|---|
ISIRLfor | GTA GAA TTC GGA TAA AGT CCG TAT AAT TGT GT |
ISIRRrev | TGT TCT AGA TGC TAT ACA ATG TTT TTA CCA TTT C |
IS91rev | CTT TTT ACT TTT ACA CAA TTA TAC GG |
IS141for | CCC AGG AGG ACT TTT ACA TGA C |
Spc-for_HindIII | ACG AAG CTT AGT CAA GTC CAG ACT CCT GTG TAA AAT CGT CCA ATC TAG GGT A |
Spc-rev_XbaI | AAG TCT AGA TGA ATT TCA CAA GAG GAC |
guaAfor | CAT GTA TCT TTG TAG ACC ATG |
guaArev | AGG ATC TGA AAC ACC TTT TAA T |
guaA_forMH | TTC GTG AAA TGG GCG TTT AT |
guaA_revMH | TAG ACT ACG CGG TTG ACG TG |
hemY_forMH | GTG ACT AAA TCA GTG GCT ATT |
hemY_revMH | TGC GAT TAC TTC TTC AGC |
hemLBDCA_for | GTT GAT CCG ATT TGC ACA C |
hemLBDCA_int1 | CCT AAT GGT GCT ATA TGA TCC |
hemLBDCA_int2 | CGT ACT GGA CTA TTT ACA CC |
hemLBDCA_int3 | GTT GCC TGT TGA ATA ACA CC |
hemLBDCA_int4 | GCG CTG TCT TAC TTC CTA TC |
hemLBDCA_int5 | CAA TAA TAC TGC CTT CTG GC |
hemLBDCA_int6 | AAC TTA ACA CAG CTA GAC CG |
hemLBDCA_int7 | ATC CAC TCA TTG TGT GCA TG |
hemLBDCA_rev | GGA TGT TCC AGG GTA TGG AT |
hemQ_for | ATT GTA TAG AGT AGC GAC TGT |
hemQ_rev | GAA CCT TAG TCT CAT CAC CT |
hemN_for | TGT CAA TGC TGA TGA TAC ATC |
hemN_rev | AGG ACA TAT TAC ATC TGG CT |
hemYHE_for | CAT CTG TCA TCT CAT TCT CTC C |
hemYHE_int1 | ATG CCA ATA CAA CAG TTG C |
hemYHE_int2 | ATT CAG GCC CTA GTT CAA TC |
hemYHE_int3 | ATT GCT TCA GTA ATG CCA TC |
hemYHE_rev | GTT GAT GGG TCG GGT TAT TG |
hemYHE_int2.5.1 | GGT ACG GAA CTC GTT ATT TC |
hemYHE_int2.5.2 | CAC AGG AGT CTG GAC TTG AC |
SA2050_for | TAT CGC TTT ATG TTA TTT CG |
SA2050_int1 | TCC TAT ACC TGC TGA AAC AG |
SA2050_int2 | TAC CGC TTC TTT CCC TTT AC |
SA2050_int3 | GCG TGC TTG GTC TTT ATA TG |
SA2050_rev | CCT CAA CAG TAC AAT GAA ATA G |
377504 F | TGT CAC GAA CCG CTA CAA CT |
378517R | TGC GAT ACC GGA AGC TTC AA |
1,112,585 F | GTT CAT CCA TCA CCA GGG CA |
1,116,051 R | TAC CAT CAC CAG CAT TCG CA |
1113415_int1 | CCA GAG AGA CGT CAA AGA C |
1114264_int2 | CTT GTA GCA ACT TGT CAC AC |
1114821_int3 | CCT GGA GAT ATG AAA GGT TAC |
Restriction sites used for cloning are underlined.