TABLE 1.
MICs of AxyXY-OprZ antibiotic substrates for Achromobacter isolates and their mutants
| Drug | MIC (μg/ml)a |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| AXX-A | AXX-A-ΔZ | ACH-CF-490 | ACH-CF-911 | CIP-102236 | CIP-102236V29G | CIP-102236R105S | ACH-NCF-39 | ACH-NCF-39V29G | ACH-CF-842 | ACH-CF-842V29G | |
| Tobramycin | 16 | 128 (8) | 32 | 64 (2) | 32 | 256 (8) | >256 (>8) | 24 | 96 (4) | 8 | 32 (4) |
| Amikacin | 256 | >256 | ≥256 | >256 | >256 | >256 | >256 | 192 | >256 (>1.3) | 32 | >256 (>8) |
| Gentamicin | 48 | >256 (>5.3) | 48 | 256 (5.3) | 192 | >256 (>1.3) | >256 (>1.3) | 48 | >256 (>5.3) | 12 | ≥256 (≥21) |
| Netilmicin | 24 | >256 (>10.7) | 64 | ≥256 (≥4) | >256 | >256 | >256 | 64 | >256 (>4) | 16 | >256 (>16) |
| Ceftazidime | 4 | 4 | 4 | 4 | 1.5 | 4 (2.6) | 3 (2) | 1.5 | 3 (2) | 1.5 | 1.5 |
| Cefepime | 16 | 128 (8) | 192 | >256 (>1.3) | 48 | >256 (>5.3) | >256 (>5.3) | 24 | >256 (>10) | 8 | 32 (4) |
| Imipenem | 1 | 1 | 2 | 4 (2) | 2 | 2 | 2 | 6 | 8 (1.3) | 2 | 4 (2) |
| Meropenem | 0.064 | 0.064 | 0.064 | 6 (94) | 0.094 | 0.125 (1.3) | 0.125 (1.3) | 0.094 | 0.125 (1.3) | 0.75 | 0.75 |
| Doripenem | 0.25 | 0.25 | 0.25 | 2 (8) | 0.38 | 0.5 (1.3) | 0.5 (1.3) | 0.25 | 0.38 (1.5) | 1 | 1 |
| Ofloxacin | 2 | 12 (6) | ≥32 | >32 | 16 | >32 (>2) | >32 (>2) | 6 | >32 (>5.3) | 24 | >32 (>1.3) |
| Levofloxacin | 1 | 4 (4) | 3 | >32 (>10) | 3 | 12 (4) | 32 (>10) | 1.5 | 6 (4) | 4 | 8 (2) |
| Ciprofloxacin | 0.75 | 2 (2.6) | 1.5 | >32 (>21) | 2 | 8 (4) | >32 (>16) | 1.5 | 8 (5.3) | 2 | 6 (3) |
| Tetracycline | 48 | 64 (1.3) | 192 | >256 (>1.3) | 96 | 128 (1.3) | >256 (>2.7) | 192 | ≥256 (≥1.3) | 48 | 192 (4) |
| Doxycycline | 8 | 12 (1.5) | 24 | 96 (4) | 12 | 16 (1.3) | 32 (2.7) | 16 | 24 (1.5) | 6 | 8 (1.3) |
| Tigecycline | 4 | 8 (2) | 3 | 3 | 2 | 2 | 3 (1.5) | 1.5 | 2 (1.3) | 0.38 | 0.5 (1.3) |
| Erythromycin | 128 | 256 (2) | 192 | >256 (>1.3) | 96 | >256 (>2.7) | >256 (>2.7) | 32 | 128 (4) | >256 | >256 |
a
Values in parentheses represent relative MIC increases (ratio of the MIC for the mutant isolate to the MIC for the original isolate).