TABLE 4.
Primers used in this study
| Primer | Nucleotide sequence (5′–3′)a |
|---|---|
| axyZ-F | ATGCGCTTGGACAGYGACGA |
| axyZ-R | CGGATRCGARTGCGAGCGTT |
| Screen-deltaAxyZ-F | TTGGAAGGAATATGGCAACG |
| Screen-deltaAxyZ-R | ACGTAGATCGGGTCGAGTTG |
| Seq-deltaAxyZ-1 | CCCTCAAGCAACTCGAAGC |
| Seq-deltaAxyZ-2 | GCTTCGAGTTGCTTGAGGG |
| Seq-deltaAxyZ-3 | CGCCAGCATACTTTTGAACA |
| Seq-deltaAxyZ-4 | TGTTCAAAAGTATGCTGGCG |
| axyY-qPCR-F3 | TGTATTCGGTCAACGGCTTC |
| axyY-qPCR-R4 | GCGCGAACACCATCAGGTT |
| rpsl-qPCR-1F | ACGCACTTTGGCAACCTTAC |
| rpsl-qPCR-1R | GCGAAGTCAGCATCATCAAA |
| rpoD-qPCR-F1 | AAGGACGGCTACAAGTCGGA |
| rpoD-qPCR-R1 | GCCAGCTTCTCGACCATCTT |
a
The nucleotide base code for degenerate primers is as follows: Y is C or T; R is A or G.