TABLE 1.
Validation data for penicillin G in plasma and dried blood spots
| Parameter | Value(s) | |||
|---|---|---|---|---|
| Penicillin G concn (mg/liter) | 0.1 | 1 | 10 | 100 |
| Plasma | ||||
| % matrix effect (± SD); n = 5) | 85.5 (8.4) | 106.24 (5.2) | 110.84 (8.9) | 94.5 (3.5) |
| % process efficiency (± SD); n = 5 | 86.4 (5.2) | 116.2 (8.3) | 107.8 (3.7) | 89.8 (6.4) |
| % absolute recovery (± SD); n = 5 | 101.5 (6.8) | 109.6 (9.5) | 97.7 (8.1) | 95.8 (5.3) |
| Interday variation (RSD%)a; n = 5 | 6.33 | 5.34 | 3.96 | 4.79 |
| Intraday variation (RSD%); n = 15 | 6.64 | 5.97 | 3.74 | 5.63 |
| Accuracy (%); n = 15 | 93.5 | 107.25 | 102.4 | 111.54 |
| Red cell partition ratio (± SD)b | 0.98 (±0.02) | 0.95 (±0.08) | 1.04 (±0.11) | 0.96 (±0.11) |
| Dried blood spots | ||||
| % matrix effect (± SD); n = 5) | 95.5 (4.3) | 84.8 (2.9) | 88.2 (11.1) | 82.1 (3.3) |
| % process efficiency (± SD); n = 5 | 101.5 (5.5) | 92.5 (4.5) | 82.6 (2.4) | 87.1 (5.4) |
| % absolute recovery (± SD); n = 5 | 107.1 (8.8) | 109.1 (4.9) | 94.5 (10.4) | 106.3 (7.2) |
| Interday variation (RSD%); n = 5 | 9.73 | 8.37 | 8.63 | 5.33 |
| Intraday variation (RSD%); n = 15 | 7.33 | 7.20 | 9.05 | 4.13 |
| Accuracy (%); n = 15 | 116 | 87.5 | 92.34 | 101.8 |
a
RSD%, percent relative standard deviation.
b
Calculated using the following formula: