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. 2017 Jul 25;61(8):e00680-17. doi: 10.1128/AAC.00680-17

FIG 2.

FIG 2

Effect of CAMs and nucleos(t)ide analogs on intracellular HBV RNA and DNA. (A and B) Uninfected and infected HepaRG cells were treated with DMSO or 30 μM BAY 41-4109_IE, BAY 41-4109, LMV, TFV, or NVR 3-1983 for 6 days prior to Northern blot analysis of intracellular ribosomal 18S and 28S RNA (A, top), HBV pgRNA and preS/S mRNA transcripts (A, middle), and encapsidated pgRNA (A, bottom) and Southern blot analysis of intracellular encapsidated HBV DNA (B). (C to F) Infected HepaRG cells were treated with increasing concentrations of NVR 3-1983 or LMV for 6 days, prior to measuring the amonts of intracellular HBV DNA (C, E) or encapsidated HBV RNA (●), total HBV mRNA transcripts (■), and beta-actin RNA (▲) (D, F). (G and H) Effect of 10 μM LMV or BAY 41-4109 on HBV cccDNA (G) and secreted HBsAg (H). Data points and error bars represent means and standard deviations from at least three independent studies, respectively.