FIG 4.

Effect of inhibitors against extracellular HBV DNA, HBV RNA, and HBsAg. Infected HepaRG cells (HRG) were treated with increasing concentrations of NVR 3-1983 (A), BAY 41-4109 (B), or LMV (C, D) for 6 days. The levels of HBV RNA (●), HBV DNA (■), and HBsAg (▲) from the cell culture supernatants were measured. (E) Primary human hepatocytes (PHH) infected with HBV were treated with either 30 μM NVR 3-1983 or 10 μM LMV for 6 days prior to determining the levels of extracellular HBV DNA (black bars) and HBV RNA (gray bars). HBV DNA and RNA levels were monitored by branched DNA and QuantiGene assays, respectively. HBsAg was measured by enzyme-linked immunosorbent assay. Data points and error bars represent means and standard deviations from at least three independent studies, respectively.