TABLE 2.
HBV RNA spliced variants detected in supernatants of infected HepaRG cells treated with and without LMV, as well as patient sera infected with HBV of genotypes A to D
| Spliced variant [size of deletion(s) (bp)] | No. of sequenced clones containing spliced variantsa |
Reference(s)b | ||||||
|---|---|---|---|---|---|---|---|---|
| HRG-S |
Patient samples |
Total | ||||||
| DMSO | LMV | 3.4 | 3.7 | 3.56 | 3.57 | |||
| Sp1 (1,223) | 3 | 3 | 3 | 4 | 5 | 8 | 26 | 29–34, 41 |
| Sp2 (282, 1,223) | 0 | 0 | 0 | 4 | 0 | 0 | 4 | 29, 30 |
| Sp3 (1,602) | 5 | 5 | 8 | 0 | 5 | 0 | 23 | 29–31 |
| Sp6 (1,199) | 2 | 1 | 0 | 0 | 0 | 0 | 3 | 29–31, 34 |
| Sp7 (455, 684) | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 29, 30 |
| Sp8 (282, 454, 688) | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 29, 30 |
| Sp9 (1,016) | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 29, 30 |
| Sp15 (168, 1,223) | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 29 |
| Sp18 (2,266) | 2 | 0 | 0 | 0 | 0 | 0 | 2 | |
| Sp19 (2,179) | 2 | 0 | 0 | 0 | 0 | 0 | 2 | |
| Sp20 (2,290) | 1 | 0 | 0 | 0 | 0 | 0 | 1 | |
| Sp21 (2,422) | 0 | 3 | 0 | 0 | 0 | 0 | 3 | |
| Total no. of clones sequenced | 16 | 12 | 11 | 11 | 11 | 11 | 72 | |
a
Variants in HepaRG cell supernatants were sequenced from 1.8- and 0.7-kb amplicons; variants in patient samples were sequenced from 1-, 1.5-, and 2-kb amplicons. HepaRG cell samples (HRG-S) were infected with genotype D HBV; patient samples 3.4, 3.7, 3.56, and 3.57 were infected with genotype B, C, D, and A HBV, respectively.
b
The reference(s) for the previously published HBV RNA or DNA variants.