FIG 2.
SAR114 and 11H10 share an overlapping epitope on ClfA001 genotype. SAR114 and 11H10 compete for binding to the ClfA001 genotype. (A) ELISA competition binding. Serial dilution (1:2 or 1:600) of SAR114 (■) or 11H10 (■) was added to ClfA-coated plates, followed by addition of biotinylated 11H10 (1:600). Percent competition was calculated as 100 × (ODMAb + 11H10biot/OD11H10biot). Data represent the mean values ± standard deviations (SD). (B) Octet binding. SAR114 (•) or 11H10 (■), diluted at 5 μg/ml, was captured on an APS biosensor and blocked with BSA, followed by addition of ClfA001 (2.5 μg/ml), and then a second MAb diluted at 5 μg/ml was added. Real-time measurement of the binding to the biosensors (in nanometers) was registered.