TABLE 3.
SAR114- and 11H10-BiS molecule affinity to ClfA variants
| MAb and ClfA variant | Affinity to ClfAa |
||
|---|---|---|---|
| Kon (M−1s−1) | Koff (s−1) | Kd | |
| SAR114-BiS2 | |||
| ClfA001 | 1.04E+06 | 5.48E−06 | 5.283 pM |
| ClfA002 | 2.66E+06 | 9.69E−05 | 36.38 pM |
| ClfA004 | 3.16E+06 | 5.74E−06 | 1.815 pM |
| 11H10-BiS2 | |||
| ClfA001 | 1.79E+06 | 5.00E−02 | 424.3 nM |
| ClfA002 | ND | ||
| ClfA004 | 9.68E+04 | 4.00E−02 | 416.5 nM |
| SAR114-BiS3 | |||
| ClfA001 | 1.64E+06 | 1.56E−05 | 9.5 pM |
| ClfA002 | 1.85E+06 | 1.13E−04 | 61.1 pM |
| ClfA004 | 6.58E+05 | 3.40E−05 | 51.6 pM |
| 11H10-BiS3 | |||
| ClfA001 | 4.60E+05 | 2.37E−02 | 51.4 nM |
| ClfA002 | 4.35E+03 | 1.26E−03 | 289.9 nM |
| ClfA004 | 1.39E+05 | 5.63E−03 | 40.3 nM |
a
The binding affinity constant (Kd) of BiS molecules to the three ClfA variants was measured by Biacore as the ratio between dissociation (Koff) and association (Kon) kinetic rate constants. ND, not determined.