TABLE 2.
Primers used for amplifying β-lactamase genes, plasmid-mediated quinolone resistance genes, and colistin resistance genes
| Primer name | Sequence (5′–3′) | Reference |
|---|---|---|
| β-Lactamase genes | ||
| blaTEM-F | TCGGGGAAATGTGCG | 26 |
| blaTEM-R | TGCTTAATCAGTGAGGCACC | |
| blaSHV-F | GCCTTTATCGGCCTTCACTCAAG | 26 |
| blaSHV-F | TTAGCGTTGCCAGTGCTCGATCA | |
| blaCTX-M-1-F | CAGCGCTTTTGCCGTCTAAG | 26 |
| blaCTX-M-1-R | GGCCCATGGTTAAAAAATCACTGC | |
| blaCTX-M-2-F | CTCAGAGCATTCGCCGCTCA | 26 |
| blaCTX-M-2-R | CCGCCGCAGCCAGAATATCC | |
| blaCTX-M-8-F | ACTTCAGCCACACGGATTCA | 26 |
| blaCTX-M-8-R | CGAGTACGTCACGACGACTT | |
| blaCTX-M-9-F | GTTACAGCCCTTCGGCGATGATTC | 26 |
| blaCTX-M-9-R | GCGCATGGTGACAAAGAGAGTGCAA | |
| Plasmid-mediated quinolone resistance genes | ||
| qnrA-F | AAGGAAGCCGTATGGATA | 27 |
| qnrA-R | AGCTAATCCGGCAGCACTAT | |
| qnrB-F | CGACCTGAGCGGCACTGAAT | 27 |
| qnrB-R | TGAGCAACGATGCCTGGTAG | |
| qnrC-F | GGGTTGTACATTTATTGAATC | 27 |
| qnrC-R | TCCACTTTACGAGGTTCT | |
| qnrD-F | CGAGATCAATTTACGGGGAATA | 27 |
| qnrD-R | AACAAGCTGAAGCGCCTG | |
| qnrS-F | ACCTTCACCGCTTGCACATT | 27 |
| qnrS-R | CCAGTGCTTCGAGAATCAGT | |
| aac(6′)-Ib-F | TGACCTTGCGATGCTCTATG | 27 |
| aac(6′)-Ib-R | TTAGGCATCACTGCGTGTTC | |
| qepA-F | CGGCGGCGTGTTGCTGGAGTTCTT | 28 |
| qepA-R | CCGACAGGCCCACGACGAGGATGC | |
| oqxA-F | CTCGGCGCGATGATGCT | 29 |
| oqxA-R | CCACTCTTCACGGGAGACGA | |
| oqxB-F | TTCTCCCCCGGCGGGAAGTAC | 29 |
| oqxB-R | CTCGGCCATTTTGGCGCGTA | |
| Colistin resistance genes | ||
| mcr-1-F | CGGTCAGTCCGTTTGTTC | 7 |
| mcr-1-R | CTTGGTCGGTCTGTAGGG | |
| mcr-2-F | TGTTGCTTGTGCCGATTGGA | 13 |
| mcr-2-R | AGATGGTATTGTTGGTTGCTG |