FIG 2.
Structures of l-captopril (A) and inhibitor 2a (B). IC50s of l-captopril (C) and inhibitor 2a (D) were calculated by fitting the data to a dose-response curve (also see Table 3). Sensorgrams for the interaction of l-captopril (E) and inhibitor 2a (F) with TMB-1, with the concentrations of the inhibitors indicated to the right. The sensorgrams for binding of inhibitor 2a to TMB-1 were fitted to a 1:1 binding model (red dotted lines). (G) Steady-state plot for binding of l-captopril to TMB-1. The KD value was determined by calculating the steady-state values from the sensorgrams, plotted as a function of the l-captopril concentration and fitted to a single-binding-site model.