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Liquid chromatography gradient programsa
| Initial program |
Modified program |
||||
|---|---|---|---|---|---|
| Time (min) | % A | % B | Time (min) | % A | % B |
| 0.00 | 0.00 | 100 | 0.00 | 30 | 70 |
| 0.57 | 0 | 100 | 1.57 | 30 | 70 |
| 1.57 | 95 | 5 | 3.00 | 40 | 60 |
| 4.00 | 95 | 5 | 4.00 | 95 | 5 |
| 5.00 | 0 | 100 | 8.00 | 95 | 5 |
| 8.00 | 0 | 100 | 9.00 | 30 | 70 |
| 11.00 | 30 | 70 | |||
Reservoir A contained LC/MS methanol; reservoir B contained 0.05% acetic acid in HPLC H2O; the HPLC column was Fortis Diphenyl, 50 by 2.1 mm (inner diameter) by 5 μm; the flow rate was 0.425 ml/min.
