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RTD-1 reduces spontaneous inflammatory cytokines in airway leukocytes. Isolated CF airway leukocytes were cultured in the presence or absence of 100 μg/ml RTD-1 for 24 h. Release of cytokines IL-1β, TNF, CXCL8, and IL-6 was quantified by ELISA. Geometric means and 95% CIs (n = 6/group) are shown. Treatment differences (log2) were analyzed by a paired t test after baseline correction (RTD-1/baseline); *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus CF leukocytes alone.
