TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, plasmid, or primer | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus | ||
| RN6390 | 8325-4 wild type | 14 |
| QT21 | 8325-4 ΔtetR21 | 1 |
| QT18 | 8325-4 ΔlmrS | This study |
| QT1821 | 8325-4 ΔlmrS ΔtetR21 | This study |
| RN6390 (pSK950-lmrS) | lmrS overexpressor | This study |
| E. coli | ||
| DH10B | E. coli for cloning | 15 |
| Top10 | F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Strr)a endA1 λ− | Invitrogen |
| DH5α (pLZ113-lmrS) | lmrS overexpressor | This study |
| DH5α (pLZ113-tetR21) | tetR21 overexpressor | This study |
| Plasmid | ||
| pSK950 | E. coli/S. aureus temp-sensitive plasmid | 16 |
| pIMAY | E. coli/S. aureus temp-sensitive plasmid | 15 |
a
Strr, streptomycin resistant.