TABLE 3.
Relative expression of efflux pump and candidate efflux pump genes in the tetR21 mutant
| S. aureus ORF | Gene | Changes in expression level (QT21 vs RN6390)a |
|
|---|---|---|---|
| 2 h | 5 h | ||
| SAOUHSC_00099 | tet38 | 4.0 ± 0.2b | 3.9 ± 0.2b |
| SAOUHSC_02762 | norD | 0.86 ± 0.2 | 1.3 ± 0.1 |
| SAOUHSC_02700 | mdeA | 0.75 ± 0.1 | 0.80 ± 0.1 |
| SAOUHSC_02418 | lmrS | 4.0 ± 0.1b | 3.9 ± 0.1b |
| SAOUHSC_02420 | sdrM | 3.2 ± 0.1b | 3.0 ± 0.1b |
| SAOUHSC_02419 | sepA | 0.9 ± 0.1 | 0.8 ± 0.1 |
| SAOUHSC_00246 | HPc | 0.9 ± 0.1 | 1.3 ± 0.1 |
| SAOUHSC_02740 | HP | 0.8 ± 0.1 | 1.0 ± 0.1 |
a
Normalized values of the tetR21 mutant QT21 over values of the wild-type RN6390 strain. The housekeeping gene gmk was used as the internal control. Each assay was done in triplicate with three separate biological samples.
b
The differences in the gene expression levels between QT21 and RN6390 are statistically significant as determined by a Student's t test (P < 0.05).
c
HP, hypothetical protein.