TABLE 2.
Primers used for candidate gene overexpression
| Gene | Primer orientation | Primer sequence (5′→3′)a |
|---|---|---|
| Rv0191 | Forward | GCTCTAGACAGATCAGCGCCGTCAC |
| Reverse | CCCAAGCTTTTAGCCGTCGCCGGG | |
| Rv3756c | Forward | GCTCTAGAACTGCCGCTGTCTATCCC |
| Reverse | CCCAAGCTTCTACCGTAGGGCGTGTC | |
| Rv3008 | Forward | GCTCTAGAGCCTGGGTTGTCACCAC |
| Reverse | CCCAAGCTTTTAGTTGCCGTCCGCGG | |
| Rv1667c | Forward | GCTCTAGACGTGGAGCTGGCCAAG |
| Reverse | CCCAAGCTTTCATTCGAGCATCTCCGAAAG |
a
Primers were flanked with recognition sequences of restriction enzymes XbaI and HindIII at their 5' end with 2 or 3 additional bp at the extreme 5' end in both primers. The forward primers of genes were taken about 300 bp upstream of the start codon to include their own promoters. The reverse primers of genes were taken 15 to 25 bp from the stop codon.