FIGURE 1.

EZH2 is required for B and T cell development. (A) Flow cytometry analysis showing B220 versus CD43 on bone marrow B220⁺CD19⁺ cells from Il7ra^cre/+ and Il7ra^cre/+Ezh2^fl/fl mice. The gated regions show pro-B cells (red, CD19⁺B220⁺CD43⁺) and mature B cells (blue, CD19⁺B220^hi). (B) The bargraph shows the mean number of pro-B and B220^hi mature B cells in the bone marrow of Il7ra^cre/+ (black) and Il7ra^cre/+Ezh2^fl/fl (white) mice. (C) Flow cytometry analysis for CD45.1 and CD45.2 on pro-B cells and B220^hi mature B cells from the bone marrow of competitive bone marrow chimeric mice 10 weeks post-reconstitution. Chimeras were established using a 1:1 ratio of bone marrow cells from CD45.1 WT B6 and CD45.2 Il7ra^cre/+Rosa^YFP (top) or Il7ra^cre/+Ezh2^fl/flRosa^YFP (bottom). (D) Flow cytometry analysis for CD4 versus CD8 on total thymocytes or CD117 versus CD25 on Lin thymocytes from Il7ra^cre/+ or Il7ra^cre/+Ezh2^fl/fl mice. (E) The bargraphs show the mean number of CD4, CD8, DP (CD4⁺CD8⁺), ETP (LinCD117⁺CD25), DN2 (LinCD117⁺CD25⁺), DN3 (LinCD117CD25⁺) and DN4 (LinCD117CD25) cells in the thymus of Il7ra^cre/+ (black) and Il7ra^cre/+Ezh2^fl/fl (white) mice. (F) Flow cytometry analysis showing CD45.1 versus CD45.2 on DN3 and DP thymocytes from competitive chimeric mice as in C. Data are representative of at least three independent experiments (mean ± SD). * p<0.05, ** p< 0.01, *** p< 0.001