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. Author manuscript; available in PMC: 2018 Jun 15.
Published in final edited form as: J Immunol. 2017 May 10;198(12):4682–4691. doi: 10.4049/jimmunol.1700319

FIGURE 2.

FIGURE 2

EZH2 is not required for NK cell or ILC2 development. (A) Flow cytometry analysis showing NK1.1 versus DX5 on Lin cells in the bone marrow and spleen of Il7racre/+ and Il7racre/+Ezh2fl/fl mice. Mature NK cells (LinDX5+NK1.1+) are shown in the gated regions. The bargraphs show the mean number of mature NK cells in the bone marrow and spleen of Il7racre/+ (black) and Il7racre/+Ezh2fl/fl (white) mice. (B) Flow cytometry analysis showing NK1.1 versus CD122 on Lin cells and CD45.1 versus CD45.2 on the gated Lin or TCRβCD19 CD122+NK1.1+ NK cells in the bone marrow (left) and spleen (right) of competitive chimeric mice as in Fig. 1C. (C) Flow cytometry analysis showing Sca1 versus CD127 on Lin cells from the bone marrow of Il7racre/+ or Il7racre/+Ezh2fl/fl mice. The gated region shows ILC2P (LinScalhiCD127+). The bargraphs show the mean number (top) or frequency (bottom) of ILC2P among Lin cells in the bone marrow of Il7racre/+ (black) and Il7racre/+Ezh2fl/fl (white) mice. (D) Flow cytometry analysis for ILC2P in the bone marrow of competitive chimeric mice as in Fig. 1C. Data are representative of at least three (A, C), five (B), or six (D) independent experiments (mean ± SD). * p < 0.05, ** p < 0.01, *** p < 0.001.