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. 2017 Jul 14;11:206. doi: 10.3389/fncel.2017.00206

Figure 3.

Figure 3

Immunofluorescence staining for NLRP6 after ICH. (A–C) Immunofluorescence staining for NeuN (A) and NLRP6 (B); the merged image shows that little NLRP6 was expressed in NeuN-positive neurons (C; arrow head). (D–F) Immunofluorescence staining for glial fibrillary acidic protein (GFAP) (D) and NLRP6 (E); the merged image shows that NLRP6 staining was also observed in the cytoplasm and end processes of astrocytes (F; arrow). (G–I) Immunofluorescence staining for CD11b (G) and NLRP6 (H); the merged image shows that NLRP6 was not expressed in CD11b-positive microglia (I). (J–L) Immunofluorescence staining for CD31 (J) and NLRP6 (K); the merged image shows that NLRP6 was not expressed in CD31-positive endothelial cell (L). All images were captured in the perihematomal brain tissues. (M) Densitometric quantification of NLRP6 expression in the perihematomal brain tissues at 1 day after ICH (n = 4 per group, **P < 0.01). Scale bars = 25 μm (A–I); = 15 μm (J–L).