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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: J Acquir Immune Defic Syndr. 2017 Aug 15;75(5):588–596. doi: 10.1097/QAI.0000000000001449

Figure 3. THC directly suppresses IFNα secretion in highly purified pDCs.

Figure 3

pDCs were isolated from PBMC via MACS (Mitenyi Biotec©). Highly purified pDCS (>95% purity) were then treated with 1, 5, 10, or 15µM THC for 30 min followed by stimulation with CpG-ODN for 5 hrs. A) FACS scatter plot of CpG-ODN induced IFNα and concentration dependent suppression by THC. B) IFNα+ pDC normalized to 0µM THC + CpG (N=5). Asterisks indicate significant differences compared to 0 µM THC + CpG (1-Way ANOVA with Dunnett’s Posttest). C) Amount of Secreted IFNα as determined by Legendplex™ secretion kit by BioLegend utilizing 1×105 isolated pDC (N=4) per treatment, treated with VC (0.026% EtOH), VC + CpG, or CpG+THC (15µM). Asterisks indicate statistically significant differences of treatment compared to 0 THC + CpG (1-way ANOVA with Dunnett’s posttest). *=p<0.5, **=p<0.01, ***=p<0.001