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. 2005 Feb 28;102(10):3587–3592. doi: 10.1073/pnas.0407170102

Fig. 3.

Fig. 3.

Functional validation of putative GW400426 targets. (a) Western blot analysis quantitating Orc6 phosphoisoforms present in Cdk1-as1 or YRP1 cells after treatment for 15 min with DMSO, 1-NA-PP1, or GW400426. (b) Representative fields of cells after treatment for 15 min of Pho85-as1 or YRP1 cells expressing a Pho4-GFP fusion construct with 5 μM 1-NA-PP1, 1% DMSO, or 20 μM GW400426. The percentage of fluorescent cells with nuclear-localized Pho4-GFP is quantified on the right. (c) Transcripts that are repressed most strongly by GW400426 or treatment of a Cdk1-as1/Pho85-as1 strain are highly enriched in G1 cell-cycle-regulated genes. Highlighted genes represent those with known roles in polarized cell growth. Quantification of mean expression ratios from each column are shown at the bottom (lanes are equivalent to those in Fig. 2a).