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. 2008 Oct 7;2(4):317–326. doi: 10.1016/j.molonc.2008.09.006

Figure 8.

Figure 8

Effect of the combination of bortezomib and curcumin on IL‐6/sIL‐6R‐induced JAK/STAT and MAPK pathways in U266 cells. U266 cells were pretreated with either bortezomib (1nM) or curcumin (5μM) or combined bortezomib and curcumin for 1h and then treated with IL‐6 (5ng/mL) and sIL‐6R (25ng/mL) for 4h. Whole‐cell extracts (30μg) were analyzed by Western blot for phosphorylated STAT3, total STAT3, phosphorylated Erk, and total Erk. Lane: 1. control; 2. 5ng/mL IL‐6 and 25ng/mL sIL‐6R; 3. 5μM curcumin, 5ng/mL IL‐6 and 25ng/mL sIL‐6R; 4. 1nM bortezomib, 5ng/mL IL‐6 and 25ng/mL sIL‐6R; 5. 5μM curcumin, 1nM bortezomib, 5ng/mL IL‐6 and 25ng/mL sIL‐6R.