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. 2008 Sep 16;2(4):349–355. doi: 10.1016/j.molonc.2008.09.001

Figure 3.

Figure 3

Detection of EGFR variants in CRC patients, patients with Crohn's disease and ulcerative colitis. Amplified cDNA fragments of EGFR wild‐type and different variants were analyzed by capillary electrophoresis with the Bioanalyzer 2100 and the DNA 1000 assay (Agilent Technologies, Böblingen, Germany). For positive control cDNA of a colorectal cancer cell line (T84) was used (Lankiewicz et al., in press). L, ladder; bp, base pairs; PCR−, negative control of the PCR; C+, positive control of the PCR; CD, Crohn's disease; UC, ulcerative colitis; CRC, colorectal cancer patients, the numbers represent the patients’ ID; a, after therapy; b, before therapy; wt, wild‐type. Patient CRC 3b dropped out before completing therapy.